I image with a 10" f/8 Meade LX850 OTA and an Optec Gemini focuser. If you have 1 micron steps, I would start at 250 steps between data points, 9 data points, 2 as the min star diameter; and 2x2 binning, smart focus disabled and 8 sec Lum exposures.
You will want to experiment with 3x3 binning, as well. For me, I needed to increase the number of steps between data point at 3x3 and increased the min star size. At 3x3 the total focusing time was cut by about 20%.
Basically looking for the worst HFR to be about 3x the best HFR. My Gemini has 0.1 micron steps, so I use 2500 steps between data points:
I have noticed that when seeing deteriorates, the V curve is much less precise but might be tweakable with changed parms during poor seeing. So, this is something I plan to experiment with in the near future.